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|a pubmed24n0370.xml
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|a (DE-627)NLM110938518
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|a (NLM)11181715
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Quartacci, M F
|e verfasserin
|4 aut
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|a Lipids and NADPH-dependent superoxide production in plasma membrane vesicles from roots of wheat grown under copper deficiency or excess
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|c 2001
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 02.08.2001
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|a Date Revised 18.03.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a The effects of in vivo copper on the lipid composition of root plasma membrane and the activities of membrane-bound enzymes, such as NADPH-dependent oxidases and lipoxygenase, were studied. Plants were grown in hydroponic culture for 11 d without Cu supply or in the presence of 50 microM Cu. Control plants were supplied with 0.3 microM Cu. Growth of roots was severely affected in the 50 microM Cu-grown plants, whereas roots grown in Cu-deficient solution did not show any difference in comparison with the control. The 50 microM Cu concentration caused an increase in the leakage of K(+) ions as well. Excess metal supply resulted in a decrease in the total lipid content of plasma membrane, a higher phospholipid amount and a reduction of steryl lipids (free sterols, steryl glycosides and acylated steryl glycosides). Cu depletion in the growth solution had only a slight effect on the plasma membrane lipid composition. In comparison with the control, only the excess of Cu caused a decrease in the lipid to protein ratio as well as a change in the phospholipid composition, with a lower phosphatidylcholine to phosphatidylethanolamine ratio. The degree of unsaturation of root plasma membranes decreased following the 0 Cu treatment and even more after the 50 microM Cu supply. Plasma membranes of wheat grown under metal deficiency and excess showed increased NADPH-dependent superoxide-producing oxidase activities, whereas membrane-bound lipoxygenase was not increased or activated due to Cu treatments. The consequences of changes in plasma membrane lipid composition and activated oxygen production as a result of Cu treatments are discussed
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fatty Acids
|2 NLM
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|a Lipids
|2 NLM
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|a Superoxides
|2 NLM
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|a 11062-77-4
|2 NLM
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|a NADP
|2 NLM
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|a 53-59-8
|2 NLM
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|a Copper
|2 NLM
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|a 789U1901C5
|2 NLM
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|a Lipoxygenase
|2 NLM
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|a EC 1.13.11.12
|2 NLM
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|a NADPH Oxidases
|2 NLM
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|a EC 1.6.3.-
|2 NLM
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|a Potassium
|2 NLM
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|a RWP5GA015D
|2 NLM
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|a Oxygen
|2 NLM
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|a S88TT14065
|2 NLM
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1 |
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|a Cosi, E
|e verfasserin
|4 aut
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1 |
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|a Navari-Izzo, F
|e verfasserin
|4 aut
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773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 52(2001), 354 vom: 19. Jan., Seite 77-84
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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773 |
1 |
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|g volume:52
|g year:2001
|g number:354
|g day:19
|g month:01
|g pages:77-84
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|a AR
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|d 52
|j 2001
|e 354
|b 19
|c 01
|h 77-84
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