Isolation and characterization of IDI2, a new Fe-deficiency-induced cDNA from barley roots, which encodes a protein related to the alpha subunit of eukaryotic initiation factor 2B (eIF2Balpha)

A new Fe-deficiency-inducible cDNA, IDI2, was isolated from Fe-deficient barley roots using the cDNA MACRO Array Technique. Accumulation of IDI2 transcripts in barley roots was strongly correlated with iron nutritional status. IDI2 encoded a protein with a low similarity to the alpha subunit of euka...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 51(2000), 353 vom: 06. Dez., Seite 2001-7
1. Verfasser: Yamaguchi, H (VerfasserIn)
Weitere Verfasser: Nakanishi, H, Nishizawa, N K, Mori, S
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Complementary DNA, Plant Eukaryotic Initiation Factor-2B IDI2 protein, Hordeum vulgare Peptide Initiation Factors Plant Proteins RNA, Messenger Iron E1UOL152H7
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100 1 |a Yamaguchi, H  |e verfasserin  |4 aut 
245 1 0 |a Isolation and characterization of IDI2, a new Fe-deficiency-induced cDNA from barley roots, which encodes a protein related to the alpha subunit of eukaryotic initiation factor 2B (eIF2Balpha) 
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500 |a Date Revised 13.05.2019 
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520 |a A new Fe-deficiency-inducible cDNA, IDI2, was isolated from Fe-deficient barley roots using the cDNA MACRO Array Technique. Accumulation of IDI2 transcripts in barley roots was strongly correlated with iron nutritional status. IDI2 encoded a protein with a low similarity to the alpha subunit of eukaryotic initiation factor 2B (eIF2Balpha). In addition, many hypothetical proteins homologous to IDI2 were also found in a database search. These proteins had limited similarity to eIF2Balpha as well as IDI2. It has been reported that these eIF2Balpha-like proteins (eIF2Balpha-LPs) are a family that is distinct from the eIF2Balpha/beta/delta family and widely distributed in the archaea, bacteria, and eukarya. A phylogenic analysis revealed that IDI2 is the first member of the eIF2Balpha-LP family to be found in higher plants. A possible role of IDI2 protein in regulating protein synthesis in Fe-deficient barley roots is proposed 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a DNA, Complementary  |2 NLM 
650 7 |a DNA, Plant  |2 NLM 
650 7 |a Eukaryotic Initiation Factor-2B  |2 NLM 
650 7 |a IDI2 protein, Hordeum vulgare  |2 NLM 
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650 7 |a Iron  |2 NLM 
650 7 |a E1UOL152H7  |2 NLM 
700 1 |a Nakanishi, H  |e verfasserin  |4 aut 
700 1 |a Nishizawa, N K  |e verfasserin  |4 aut 
700 1 |a Mori, S  |e verfasserin  |4 aut 
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