Extended stability of restriction enzymes at ambient temperatures

Extended stability of restriction enzymes at ambient temperatures

The stability of restriction enzymes as supplied by manufacturers without any modification has been examined. No reduction in activity was observed for three enzymes (HindIII, EcoRI and Tsp509I) held at ambient temperature or 4 degrees C for the period of study (12 months), while activity was observ...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 29(2000), 3 vom: 15. Sept., Seite 536-8, 540, 542
1. Verfasser: Clark, J (VerfasserIn)
Weitere Verfasser: Harrison, J C, Mdegela, R H, March, J B
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report Trehalose B8WCK70T7I DNA Restriction Enzymes EC 3.1.21.- Deoxyribonuclease EcoRI Deoxyribonuclease HindIII endodeoxyribonuclease XBAI CCCGGG-specific type II deoxyribonucleases mehr... EC 3.1.21.4 CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
LEADER 01000naa a22002652 4500
001 NLM109173724
003 DE-627
005 20231222150755.0
007 tu
008 231222s2000 xx ||||| 00| ||eng c
028 5 2 |a pubmed24n0364.xml 
035 |a (DE-627)NLM109173724 
035 |a (NLM)10997268 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Clark, J  |e verfasserin  |4 aut 
245 1 0 |a Extended stability of restriction enzymes at ambient temperatures 
264 1 |c 2000 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 18.01.2001 
500 |a Date Revised 28.09.2018 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a The stability of restriction enzymes as supplied by manufacturers without any modification has been examined. No reduction in activity was observed for three enzymes (HindIII, EcoRI and Tsp509I) held at ambient temperature or 4 degrees C for the period of study (12 months), while activity was observed for up to 12 weeks after storage at 37 degrees C, which was considerably better than following desiccation with trehalose, a recognized preservation technique. A larger trial of 23 different restriction enzymes held at room temperature for one week showed that all enzymes retained significant activity. As a practical demonstration of the usefulness of this finding, enzymes were posted to Africa by conventional mail (cost $1 US) and shown to retain activity upon arrival after three weeks in transit (compared to a cost of $1000 US by cold-chain transportation). Supplying enzymes to third-world markets should now be possible by removing the necessity for cold-chain transport. After arrival, enzymes can simply be stored in a standard domestic refrigerator 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Technical Report 
650 7 |a Trehalose  |2 NLM 
650 7 |a B8WCK70T7I  |2 NLM 
650 7 |a DNA Restriction Enzymes  |2 NLM 
650 7 |a EC 3.1.21.-  |2 NLM 
650 7 |a Deoxyribonuclease EcoRI  |2 NLM 
650 7 |a EC 3.1.21.-  |2 NLM 
650 7 |a Deoxyribonuclease HindIII  |2 NLM 
650 7 |a EC 3.1.21.-  |2 NLM 
650 7 |a endodeoxyribonuclease XBAI  |2 NLM 
650 7 |a EC 3.1.21.-  |2 NLM 
650 7 |a CCCGGG-specific type II deoxyribonucleases  |2 NLM 
650 7 |a EC 3.1.21.4  |2 NLM 
650 7 |a CTGCAG-specific type II deoxyribonucleases  |2 NLM 
650 7 |a EC 3.1.21.4  |2 NLM 
650 7 |a Deoxyribonucleases, Type II Site-Specific  |2 NLM 
650 7 |a EC 3.1.21.4  |2 NLM 
700 1 |a Harrison, J C  |e verfasserin  |4 aut 
700 1 |a Mdegela, R H  |e verfasserin  |4 aut 
700 1 |a March, J B  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t BioTechniques  |d 1988  |g 29(2000), 3 vom: 15. Sept., Seite 536-8, 540, 542  |w (DE-627)NLM012627046  |x 1940-9818  |7 nnns 
773 1 8 |g volume:29  |g year:2000  |g number:3  |g day:15  |g month:09  |g pages:536-8, 540, 542 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_21 
912 |a GBV_ILN_22 
912 |a GBV_ILN_39 
912 |a GBV_ILN_40 
912 |a GBV_ILN_50 
912 |a GBV_ILN_60 
912 |a GBV_ILN_62 
912 |a GBV_ILN_65 
912 |a GBV_ILN_70 
912 |a GBV_ILN_99 
912 |a GBV_ILN_121 
912 |a GBV_ILN_130 
912 |a GBV_ILN_227 
912 |a GBV_ILN_350 
912 |a GBV_ILN_618 
912 |a GBV_ILN_640 
912 |a GBV_ILN_754 
912 |a GBV_ILN_2001 
912 |a GBV_ILN_2002 
912 |a GBV_ILN_2003 
912 |a GBV_ILN_2005 
912 |a GBV_ILN_2006 
912 |a GBV_ILN_2007 
912 |a GBV_ILN_2008 
912 |a GBV_ILN_2009 
912 |a GBV_ILN_2010 
912 |a GBV_ILN_2012 
912 |a GBV_ILN_2015 
912 |a GBV_ILN_2018 
912 |a GBV_ILN_2023 
912 |a GBV_ILN_2035 
912 |a GBV_ILN_2040 
912 |a GBV_ILN_2060 
912 |a GBV_ILN_2099 
912 |a GBV_ILN_2105 
912 |a GBV_ILN_2121 
912 |a GBV_ILN_2470 
951 |a AR 
952 |d 29  |j 2000  |e 3  |b 15  |c 09  |h 536-8, 540, 542