Response differences between human CD4(+) and CD8(+) T-cells during CD28 costimulation : implications for immune cell-based therapies and studies related to the expansion of double-positive T-cells during aging
Copyright 2000 Academic Press.
| Veröffentlicht in: | Clinical immunology (Orlando, Fla.). - 1999. - 96(2000), 3 vom: 01. Sept., Seite 187-97 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2000
|
| Zugriff auf das übergeordnete Werk: | Clinical immunology (Orlando, Fla.) |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, P.H.S. CD28 Antigens HLA-DP Antigens |
| Zusammenfassung: | Copyright 2000 Academic Press. Since CD28 costimulation is critical for T-cell activation, there is great interest in CD28 as a target for immuntherapeutic approaches. We show that stimulation of human CD4(+) and CD8(+) T-cells differs in their responsiveness to stimulation with anti-CD3/CD28-coated beads, as surrogate antigen-presenting cells. While the CD4(+) subset responded with sustained proliferation, CD8(+) T-cells grew for a limited period only and failed to produce IL-2 beyond the first few days in culture. This decrease is accompanied with an increased rate of apoptosis in CD8(+) T-cells despite Bcl-x(L) expression. The CD8(+) but not the CD4(+) subset developed a reversible double-positive phenotype during CD28 costimulation. This finding may have some bearing on the appearance of double-positive T-cells in human peripheral blood. This double-positive subset was shown to undergo a statistically significantly increase during aging in humans. Taken together, the above data have important implications for immunotherapy and immune senescence |
|---|---|
| Beschreibung: | Date Completed 22.09.2000 Date Revised 16.11.2017 published: Print Citation Status MEDLINE |
| ISSN: | 1521-6616 |