Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes

Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes

An improved PCR-based subtractive hybridization strategy was used to clone apoptosis-related genes induced by all-trans retinoic acid (ATRA) from human promyelocytic leukemia cell line HL-60 cells. The protocol used the cap-finder method, long-distance PCR, streptavidin magnetic bead-mediated subtra...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 29(2000), 2 vom: 08. Aug., Seite 310-3
1. Verfasser: Zhu, F (VerfasserIn)
Weitere Verfasser: Yan, W, Zhao, Z L, Chai, Y B, Lu, F, Wang, Q, Peng, W D, Yang, A G, Wang, C J
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Technical Report Journal Article DNA, Complementary Neoplasm Proteins Tretinoin 5688UTC01R Streptavidin 9013-20-1