Technique for cloning and sequencing the ends of bacterial artificial chromosome inserts

Bacterial artificial chromosome (BAC) libraries are an important tool for positional cloning, gene analysis and physical mapping. During studies using BAC clones, it is often necessary to organize them into contiguous sequences (contigs). To finalize, join and extend the contigs, both cloning and se...

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Publié dans:BioTechniques. - 1993. - 29(2000), 2 vom: 08. Aug., Seite 271-4, 276
Auteur principal: Ripoll, P J (Auteur)
Autres auteurs: O'Sullivan, D M, Edwards, K J, Rodgers, M
Format: Article
Langue:English
Publié: 2000
Accès à la collection:BioTechniques
Sujets:Research Support, Non-U.S. Gov't Technical Report Validation Study Journal Article DNA, Bacterial DNA, Plant DNA, Recombinant Reagent Kits, Diagnostic