Technique for cloning and sequencing the ends of bacterial artificial chromosome inserts

Bacterial artificial chromosome (BAC) libraries are an important tool for positional cloning, gene analysis and physical mapping. During studies using BAC clones, it is often necessary to organize them into contiguous sequences (contigs). To finalize, join and extend the contigs, both cloning and se...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 29(2000), 2 vom: 08. Aug., Seite 271-4, 276
1. Verfasser: Ripoll, P J (VerfasserIn)
Weitere Verfasser: O'Sullivan, D M, Edwards, K J, Rodgers, M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report Validation Study DNA, Bacterial DNA, Plant DNA, Recombinant Reagent Kits, Diagnostic