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|a pubmed24n1220.xml
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|a (DE-627)NLM108667715
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|a (NLM)10944147
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Murchie, E H
|e verfasserin
|4 aut
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|a Short-term nitrogen-induced modulation of phosphoenolpyruvate carboxylase in tobacco and maize leaves
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|c 2000
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 20.09.2000
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|a Date Revised 13.12.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a Untransformed maize and tobacco plants and tobacco plants constitutively expressing nitrate reductase were grown with sufficient NO(3)- to support maximal growth. Four days prior to treatment the tobacco plants were deprived of nitrogen. Excised maize leaves and tobacco leaf discs were fed with either 40 mM KNO(3) or 40 mM KCl (control) in the light. Phosphoenolpyruvate (PEP) carboxylase (Case) activity was measured at 0.3 mM and 3 mM PEP. The light- induced increase in PEPCase V(max) was greater in maize than tobacco. Furthermore light decreased malate sensitivity in maize (which was N-replete) but not in N-deficient tobacco. NO(3)- treatment increased PEPCase V:(max) values in both species and decreased the sensitivity to inhibition by malate, but effects of NO(3)- were much more pronounced in tobacco than maize. PEPCase kinase activity was, however, greater in maize leaves NO(3)- than in the Cl(-)-treated controls, suggesting that it is responsive to leaf nitrogen supply. A correlation between foliar glutamine content and PEPCase activity was observed. It is concluded that PEPCase is sensitive to N metabolites which favour increased flow through the anapleurotic pathway in both C(3) and C(4) plants
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Nitrates
|2 NLM
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|a Phosphoenolpyruvate Carboxylase
|2 NLM
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|a EC 4.1.1.31
|2 NLM
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|a Nitrogen
|2 NLM
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|a N762921K75
|2 NLM
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|a Ferrario-Méry, S
|e verfasserin
|4 aut
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|a Valadier, M H
|e verfasserin
|4 aut
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|a Foyer, C H
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 51(2000), 349 vom: 15. Aug., Seite 1349-56
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:51
|g year:2000
|g number:349
|g day:15
|g month:08
|g pages:1349-56
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|a GBV_USEFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 51
|j 2000
|e 349
|b 15
|c 08
|h 1349-56
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