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01000naa a22002652 4500 |
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NLM108614573 |
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DE-627 |
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20231222145546.0 |
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231222s2000 xx ||||| 00| ||eng c |
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|a pubmed24n0362.xml
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|a (DE-627)NLM108614573
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| 035 |
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|a (NLM)10938793
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Schoenbeck, M A
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Decreased NADH glutamate synthase activity in nodules and flowers of alfalfa (Medicago sativa L.) transformed with an antisense glutamate synthase transgene
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| 264 |
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1 |
|c 2000
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 25.08.2000
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| 500 |
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|a Date Revised 21.11.2013
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a Legumes obtain a substantial portion of their nitrogen (N) from symbiotic N2 fixation in root nodules. The glutamine synthetase (GS, EC 6.3.1.2)/glutamate synthase (GOGAT) cycle is responsible for the initial N assimilation. This report describes the analysis of a transgenic alfalfa (Medicago sativa L.) line containing an antisense NADH-GOGAT (EC 1.4.1.14) under the control of the nodule-enhanced aspartate amino-transferase (AAT-2) promoter. In one transgenic line, NADH-GOGAT enzyme activity was reduced to approximately 50%, with a corresponding reduction in protein and mRNA. The transcript abundance for cytosolic GS, ferredoxin-dependent GOGAT (EC 1.4.7.1), AAT-2 (EC 2.6.1.1), asparagine synthase (EC 6.3.5.4), and phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) were unaffected, as were enzyme activities for AAT, PEPC and GS. Antisense NADH-GOGAT plants grown under symbiotic conditions were moderately chlorotic and reduced in growth and N content, even though symbiotic N2 fixation was not significantly reduced. The addition of nitrate relieved the chlorosis and restored growth and N content. Surprisingly, the antisense NADH-GOGAT plants were male sterile resulting from inviable pollen. A reduction in NADH-GOGAT enzyme activity and transcript abundance in the antisense plants was measured during the early stages of flower development. Inheritance of the transgene was stable and resulted in progeny with a range of NADH-GOGAT activity. These data indicate that NADH-GOGAT plays a critical role in the assimilation of symbiotically fixed N and during pollen development
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Antisense Elements (Genetics)
|2 NLM
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| 650 |
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7 |
|a NAD
|2 NLM
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| 650 |
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7 |
|a 0U46U6E8UK
|2 NLM
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| 650 |
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7 |
|a Glutamate Synthase
|2 NLM
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| 650 |
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7 |
|a EC 1.4.1.13
|2 NLM
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| 700 |
1 |
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|a Temple, S J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Trepp, G B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Blumenthal, J M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Samac, D A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gantt, J S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hernandez, G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Vance, C P
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 51(2000), 342 vom: 01. Jan., Seite 29-39
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:51
|g year:2000
|g number:342
|g day:01
|g month:01
|g pages:29-39
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 51
|j 2000
|e 342
|b 01
|c 01
|h 29-39
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