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01000naa a22002652 4500 |
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NLM108603601 |
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DE-627 |
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20231222145532.0 |
| 007 |
tu |
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231222s2000 xx ||||| 00| ||eng c |
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|a pubmed24n0362.xml
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|a (DE-627)NLM108603601
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| 035 |
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|a (NLM)10937695
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Waterworth, W M
|e verfasserin
|4 aut
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| 245 |
1 |
4 |
|a The barley scutellar peptide transporter
|b biochemical characterization and localization to the plasma membrane
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| 264 |
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1 |
|c 2000
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 06.09.2000
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| 500 |
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|a Date Revised 21.11.2013
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a Thiol-affinity labelling was used to identify and characterize components of the peptide transport system in the barley (Hordeum vulgare) scutellar epithelium. SDS-PAGE and 2D-PAGE in conjunction with fluorography were used to study derivatized proteins. Membrane proteins of 42 kDa and 66 kDa were identified using a strategy devised to label substrate protectable protein with the thiol specific reagent [14C] N-ethylmaleimide (NEM). The scutellar plasma membrane is the anticipated site of transporters involved in the mobilization of endosperm storage reserves in the germinating barley grain. The subcellular localization of these proteins to the plasma membrane was demonstrated by thiol-affinity labelling of high purity plasma membrane vesicles isolated from barley scutellar tissue. A peptide transporter, HvPTR1, specific to the barley scutellum has recently been cloned in this laboratory. A 66 kDa protein, comparable to the predicted molecular mass of HvPTR1, was identified by [14C]NEM labelling studies of Xenopus laevis oocytes expressing HvPTR1 cRNA, but not water injected controls. Peptide antiserum raised to HvPTR1 also cross-reacted with a 66 kDa membrane protein in barley scutellar tissue. This confirms that the 66 kDa protein identified here by thiol-affinity labelling studies is the barley scutellum peptide transporter HvPTR1, and demonstrates that this protein is localized to the plasma membrane of scutellar epithelial cells during germination
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Affinity Labels
|2 NLM
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| 650 |
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7 |
|a Carrier Proteins
|2 NLM
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| 650 |
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7 |
|a Membrane Transport Proteins
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Ptr1 protein, Hordeum vulgare
|2 NLM
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| 650 |
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7 |
|a Cysteine
|2 NLM
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| 650 |
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7 |
|a K848JZ4886
|2 NLM
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| 650 |
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7 |
|a Ethylmaleimide
|2 NLM
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| 650 |
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7 |
|a O3C74ACM9V
|2 NLM
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| 700 |
1 |
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|a West, C E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bray, C M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 51(2000), 348 vom: 17. Juli, Seite 1201-9
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:51
|g year:2000
|g number:348
|g day:17
|g month:07
|g pages:1201-9
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 51
|j 2000
|e 348
|b 17
|c 07
|h 1201-9
|