Method for cloning in vivo targets of the Egr-1 transcription factor
A methodology is described that allows the in vivo trapping of transcription factors to their target regulatory elements in multiple genes simultaneously. Cross-linking using formaldehyde is the first of several steps to isolate, purify, clone and characterize multiple gene promoter DNA fragments. T...
| Publié dans: | BioTechniques. - 1993. - 29(2000), 1 vom: 17. Juli, Seite 162-9 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2000
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Cross-Linking Reagents DNA-Binding Proteins EGR1 protein, human Early Growth Response Protein 1 Immediate-Early Proteins Transcription Factors Transforming Growth Factor beta plus... |