Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR
We have developed a quantitative RT-PCR method that can be used to determine the amount of enterovirus RNA in urban sludge samples. This method combines Taq-Man technology with the ABI Prism 7700 real-time sequence detection system. We optimized a one-step RT-PCR that uses a dual-labeled fluorogenic...
Veröffentlicht in: | BioTechniques. - 1988. - 29(2000), 1 vom: 17. Juli, Seite 88-93 |
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1. Verfasser: | |
Weitere Verfasser: | , , , , , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
2000
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Research Support, Non-U.S. Gov't Technical Report Fluorescent Dyes RNA, Viral Sewage |