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NLM107061414 |
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DE-627 |
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20231222142224.0 |
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tu |
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231222s2000 xx ||||| 00| ||eng c |
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|a pubmed24n0357.xml
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|a (DE-627)NLM107061414
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|a (NLM)10779406
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Rider, V
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Molecular mechanisms involved in the estrogen-dependent regulation of calcineurin in systemic lupus erythematosus T cells
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| 264 |
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1 |
|c 2000
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 16.06.2000
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Copyright 2000 Academic Press.
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|a Previous experiments in our laboratory indicated that calcineurin expression and PP2B phosphatase activity increased when estrogen was cultured with SLE T cells but not with T cells from normal women. In this report we extended our findings to show that estrogen receptor (ER) antagonism by ICI 182,780 inhibited the estrogen-dependent increase in calcineurin mRNA and phosphatase PP2B activity indicating that estrogen action was mediated through the ER. Inhibition of de novo protein synthesis with cycloheximide suggested that the estrogen-dependent increase in T cell calcineurin mRNA was a direct effect of the ER and new protein synthesis was not required. Estrogen increased calcineurin mRNA in systemic lupus erythematosus (SLE) T cells at 6 h after the start of culture correlating with increased phosphatase activity at this same time. Phosphatase activity increased significantly (P < 0.02) in lupus T cells cultured for 8 h in estradiol-containing medium. Reverse transcription and polymerase chain amplification revealed that ER-beta and ER-alpha were expressed in female and male T cells from SLE patients and normal controls. However, calcineurin steady-state mRNA levels were unaffected by estradiol in cultured T cells from male SLE patients and normal male and female controls. These data indicate that estrogen, bound to the ER, evokes a direct increase in calcineurin expression in T cells from female lupus patients. This gender-specific response suggests that ER function is altered in women with the female predominant autoimmune disease, SLE
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4 |
|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Estrogens
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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7 |
|a Receptors, Estrogen
|2 NLM
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| 650 |
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7 |
|a Estradiol
|2 NLM
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| 650 |
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7 |
|a 4TI98Z838E
|2 NLM
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| 650 |
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|a Calcineurin
|2 NLM
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| 650 |
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7 |
|a EC 3.1.3.16
|2 NLM
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| 700 |
1 |
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|a Jones, S R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Evans, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Abdou, N I
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 95(2000), 2 vom: 01. Mai, Seite 124-34
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:95
|g year:2000
|g number:2
|g day:01
|g month:05
|g pages:124-34
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| 912 |
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 95
|j 2000
|e 2
|b 01
|c 05
|h 124-34
|