Relative quantitative RT-PCR protocol for TrkB expression in neuroblastoma using GAPD as an internal control
An RT-PCR protocol for the relative quantitative measurement of TrkB transcripts using glyceraldehyde-3-phosphate-dehydrogenase (GAPD) transcripts as an internal control is described. Both TrkB and GAPD sequences were co-amplified in the exponential phase of amplification using 5'-biotinylated...
| Publié dans: | BioTechniques. - 1991. - 28(2000), 4 vom: 19. Apr., Seite 681-2, 686, 688-91 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2000
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. RNA, Messenger Glyceraldehyde-3-Phosphate Dehydrogenases EC 1.2.1.- Receptor, trkB EC 2.7.10.1 |