Restriction site-free insertion of PCR products directionally into vectors
A restriction site-free cloning method has been developed for inserting a PCR product into a vector flexibly and precisely at any desired location with high efficiency. The method uses a pair of DNA integration primers with two portions. The 3' portion isolates the inserts by PCR, and the 5...
| Veröffentlicht in: | BioTechniques. - 1991. - 28(2000), 3 vom: 14. März, Seite 498-500, 504-5 |
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| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article |