Single-molecule fluorescence observed with mercury lamp illumination

We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine,...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 27(1999), 5 vom: 22. Nov., Seite 1008-14
Auteur principal: Unger, M (Auteur)
Autres auteurs: Kartalov, E, Chiu, C S, Lester, H A, Quake, S R
Format: Article
Langue:English
Publié: 1999
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, U.S. Gov't, P.H.S. Luminescent Proteins Peptides Rhodamines Green Fluorescent Proteins 147336-22-9 polyhistidine 26062-48-6 Histidine plus... 4QD397987E Streptavidin 9013-20-1 Mercury FXS1BY2PGL