Single-molecule fluorescence observed with mercury lamp illumination
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine,...
| Publié dans: | BioTechniques. - 1991. - 27(1999), 5 vom: 22. Nov., Seite 1008-14 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1999
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, P.H.S. Luminescent Proteins Peptides Rhodamines Green Fluorescent Proteins 147336-22-9 polyhistidine 26062-48-6 Histidine plus... |