Evaluation of methods for transient transfection of a murine macrophage cell line, RAW 264.7

Monocyte/macrophage cell lines are fastidious cells commonly used in transient transfection experiments. In the course of a study of gene regulation by lipopolysaccharide (LPS), we have compared several methods for DNA-mediated cell transfection to determine which would be optimally applicable to th...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 27(1999), 4 vom: 01. Okt., Seite 824-6, 828-30, 832
1. Verfasser: Thompson, C D (VerfasserIn)
Weitere Verfasser: Frazier-Jessen, M R, Rawat, R, Nordan, R P, Brown, R T
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Calcium Phosphates Cation Exchange Resins Lipids Lipofectamine Lipopolysaccharides Phosphatidylethanolamines alpha-tricalcium phosphate tetracalcium phosphate calcium phosphate, monobasic, anhydrous mehr... 701EKV9RMN 1,2-dielaidoylphosphatidylethanolamine 76391-83-8 DNA 9007-49-2 DEAE-Dextran 9015-73-0 calcium phosphate 97Z1WI3NDX Luciferases EC 1.13.12.- beta-Galactosidase EC 3.2.1.23 calcium phosphate, dibasic, anhydrous L11K75P92J