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231222s1999 xx ||||| 00| ||eng c |
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|a pubmed25n0349.xml
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|a (DE-627)NLM104550163
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|a (NLM)10524324
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Ivanetich, K M
|e verfasserin
|4 aut
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|a Automated purification and quantification of oligonucleotides
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|c 1999
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 19.11.1999
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a We have developed automated methods for the trityl-on purification and quantification of synthetic oligonucleotides. Oligonucleotide purification is by solid-phase extraction cartridges using Amberchrom CG-50 resin on an XYZ-axis robotic system. Quantification is by OD260nm using an online UV-visible spectrophotometer with sipper. The purification of 20 oligonucleotides requires 5 min of user set-up time, plus 20 min per sample of robot time. For a 15-25-mer at the 40 nmol scale of synthesis, the method gives a yield of 2.8 ODs from a load of 10.1 OD, i.e., a 28% average yield. Oligonucleotides purified by this method have proven to be successful for primers for automated DNA sequencing
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|a Comparative Study
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|a Journal Article
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| 650 |
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7 |
|a Oligonucleotides
|2 NLM
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| 700 |
1 |
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|a Reid, R C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ellison, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Perry, K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Taylor, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Reschenberg, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mainieri, A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhu, D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Argo, J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cass, D
|e verfasserin
|4 aut
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| 700 |
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|a Strickland, C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 27(1999), 4 vom: 01. Okt., Seite 810-2, 814-8, 820 passim
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:27
|g year:1999
|g number:4
|g day:01
|g month:10
|g pages:810-2, 814-8, 820 passim
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