Co-transfected SV40 origin of replication activates expression from SV40 promoterless constructs

Co-transfection with expression plasmids is widely used to control DNA uptake efficiency in transient transfection experiments. However, a number of problems have been associated with their use. Here, we describe the activation of expression of constructs not containing the simian virus 40 (SV40) or...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 27(1999), 4 vom: 01. Okt., Seite 728-32
1. Verfasser: Flint, A (VerfasserIn)
Weitere Verfasser: Kaluz, S, Kaluzova, M, Sheldrick, L, Fisher, P, Derecka, K
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Technical Report Journal Article Receptors, Estrogen Chloramphenicol O-Acetyltransferase EC 2.3.1.28 Thymidine Kinase EC 2.7.1.21 beta-Galactosidase EC 3.2.1.23