Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size

Tyramide signal amplification (TSA)-FISH was used to map one mouse and two human DNA probes of less than 1 kb in size. The two human probes were 319 and 608 bp, and the mouse probe was 855 bp. Probes, made from PCR products, were labeled by incorporating biotin-11-dUTP (human) and biotin-16-dUTP (mo...

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Publié dans:	BioTechniques. - 1991. - 27(1999), 3 vom: 01. Sept., Seite 608-13
Auteur principal:	Schriml, L M (Auteur)
Autres auteurs:	Padilla-Nash, H M, Coleman, A, Moen, P, Nash, W G, Menninger, J, Jones, G, Ried, T, Dean, M
Format:	Article
Langue:	English
Publié:	1999
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, U.S. Gov't, P.H.S. DNA Probes Deoxyuracil Nucleotides biotin-16-dUTP biotinyltyramide Biotin 6SO6U10H04 biotin-11-dUTP 86303-25-5 plus... Tyramine X8ZC7V0OX3