Differential display probes for cDNA arrays
PCR with a combination of one arbitrary and one oligo(dT) anchor primer can be used to generate an effective probe for cDNA arrays. The method uses less than 1/200 of the amount of RNA used in some other array hybridization methods. Each fingerprint detects approximately 5% of the transcribed mRNAs,...
| Veröffentlicht in: | BioTechniques. - 1993. - 27(1999), 3 vom: 01. Sept., Seite 554-60, 562, 564 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1999
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. DNA Primers DNA Probes DNA, Complementary Oligodeoxyribonucleotides RNA, Messenger oligo (dT) mehr... |