Direct observation of nucleocytoplasmic transport by microinjection of GFP-tagged proteins in living cells
We established a straightforward experimental system to investigate directly the requirements for nucleocytoplasmic transport in live cells. For this purpose, substrates were created containing nuclear localization signals (NLS) or nuclear export signals (NES) linked to a chimeric protein composed o...
| Veröffentlicht in: | BioTechniques. - 1993. - 27(1999), 2 vom: 22. Aug., Seite 350-5 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1999
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Luminescent Proteins Nuclear Localization Signals Green Fluorescent Proteins 147336-22-9 Glutathione Transferase EC 2.5.1.18 |