pSURF-2, a modified BAC vector for selective YAC cloning and functional analysis

A modified bacterial artificial chromosome (BAC) vector, pSURF-2, adapted for the selective subcloning of yeast artificial chromosome (YAC) sequences was constructed. DH10B-U, a pyrF derivative of the highly transformable E. coli strain DH10B was also constructed and used for the detection of Ura+ r...

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Veröffentlicht in:BioTechniques. - 1993. - 27(1999), 1 vom: 01. Juli, Seite 164-70, 172, 175
1. Verfasser: Boyd, A C (VerfasserIn)
Weitere Verfasser: Davidson, H, Stevenson, B, McLachlan, G, Davidson-Smith, H, Porteous, D J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't CFTR protein, human Cinnamates DNA, Bacterial DNA, Fungal Genetic Markers Viral Proteins Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6 mehr... Hygromycin B 3XQ2233B0B hygromycin A 3YJY415DDI Monophenol Monooxygenase EC 1.14.18.1 Cre recombinase EC 2.7.7.- Integrases