Influence of additives on the protoplasts electrofusion

Various neutral or charged surface active substances were used for testing the influence of additives on the electrofusion of barley protoplasts. It was found that neutral surface active agents DX, TAGB, Span-80 and AEO-9 could promote the electrofusion. The positively charged surface active agents...

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Veröffentlicht in:Bioelectrochemistry and bioenergetics (Lausanne, Switzerland). - 1999. - 48(1999), 2 vom: 14. Mai, Seite 447-51
1. Verfasser: Jiang, Z (VerfasserIn)
Weitere Verfasser: Wang, M, Wang, W
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Surface-Active Agents
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520 |a Various neutral or charged surface active substances were used for testing the influence of additives on the electrofusion of barley protoplasts. It was found that neutral surface active agents DX, TAGB, Span-80 and AEO-9 could promote the electrofusion. The positively charged surface active agents Bardac 2080, Bardac 2280 and amphoteric surface active agents dodecyl-propyl betaine and CAB betaine also promote the electrofusion, but at high concentration the electrofusion efficiency will reduce. The negatively charged polymer agents Cibacron blue DX, Fluoresceinylthiocarbamoyl DX, and active surface substances K12 and Carsonol TLS- presented negative effect. These phenomena were discussed from the view of adsorption of additives on the membrane and the interactions between protoplasts 
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