Microsatellite analysis using a two-step procedure for fluorescence labeling of PCR products

A method for fluorescent labeling of PCR products has been developed. This method consists in a two-step procedure in which a first exponential classical PCR is followed by a "linear amplification". This second step relies on incorporation of fluorescent dNTP (dUTP or dCTP) in order to lab...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 26(1999), 5 vom: 01. Mai, Seite 902-5
1. Verfasser: Jouquand, S (VerfasserIn)
Weitere Verfasser: Chéron, A, Galibert, F
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report DNA Primers Deoxycytosine Nucleotides Deoxyuracil Nucleotides Fluorescent Dyes Phosphorus Radioisotopes deoxyuridine triphosphate 1173-82-6 2'-deoxycytidine 5'-triphosphate 2056-98-6