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231222s1999 xx ||||| 00| ||eng c |
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|a (NLM)10228578
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Yang, J
|e verfasserin
|4 aut
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|a Direct electron transfer for hemoglobin in biomembrane-like dimyristoyl phosphatidylcholine films on pyrolytic graphite electrodes
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|c 1999
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 14.06.1999
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|a Date Revised 03.11.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Stable thin films made from dimyristoyl phosphatidylcholine (DMPC) with incorporated hemoglobin (Hb) on pyrolytic graphite (PG) electrodes were characterized by electrochemical and other techniques. Cyclic voltammetry (CV) of Hb-DMPC films showed a pair of well-defined and nearly reversible peaks at about -0.27 V vs. saturated calomel electrode (SCE) at pH 5.5, characteristic of Hb heme Fe(III)/Fe(II) redox couple. The electron transfer between Hb and PG electrodes was greatly facilitated in DMPC films. Apparent heterogeneous rate constants (ks) were estimated by fitting square wave voltammograms of Hb-DMPC films to a model featuring thin layer behavior and dispersion of formal potentials for redox center. The formal potential of Hb heme Fe(III)/Fe(II) couple in DMPC films shifted linearly between pH 4.5 to 11 with a slope of -48 mV pH-1, suggesting that one proton is coupled to each electron transfer in the electrochemical reaction. Soret absorption band positions suggest that Hb retains a near native conformation in DMPC films at medium pH. Differential scanning calorimetry (DSC) showed the phase transition for DMPC and Hb-DMPC films, suggesting DMPC has an ordered multibilayer structure. Trichloroacetic acid (TCA) was catalytically reduced by Hb-DMPC films with significant decreases in the electrode potential required
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Hemoglobins
|2 NLM
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|a Membranes, Artificial
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|a Trichloroacetic Acid
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|a 5V2JDO056X
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|a Graphite
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|a Iron
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|a E1UOL152H7
|2 NLM
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|a Dimyristoylphosphatidylcholine
|2 NLM
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|a U86ZGC74V5
|2 NLM
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| 700 |
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|a Hu, N
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)
|d 1999
|g 48(1999), 1 vom: 03. Feb., Seite 117-27
|w (DE-627)NLM098241303
|x 0302-4598
|7 nnns
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| 773 |
1 |
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|g volume:48
|g year:1999
|g number:1
|g day:03
|g month:02
|g pages:117-27
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