Fast reversible electron transfer for photosynthetic reaction center from wild type Rhodobacter sphaeroides re-constituted in polycation sandwiched monolayer film

Direct reversible electron transfer for photosynthetic reaction center from wild type Rhodobacter sphaeroides re-constituted in polycation sandwiched monolayer film was observed in this work. The redox potential E0' = 0.46 V vs. NHE for first primary donor redox couple P/P+ was accurately measu...

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Veröffentlicht in:Bioelectrochemistry and bioenergetics (Lausanne, Switzerland). - 1999. - 48(1999), 1 vom: 03. Feb., Seite 101-7
1. Verfasser: Kong, J (VerfasserIn)
Weitere Verfasser: Sun, W, Wu, X, Deng, J, Lu, Z, Lvov, Y, Desamero, R Z, Frank, H A, Rusling, J F
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Membranes, Artificial Photosynthetic Reaction Center Complex Proteins
Beschreibung
Zusammenfassung:Direct reversible electron transfer for photosynthetic reaction center from wild type Rhodobacter sphaeroides re-constituted in polycation sandwiched monolayer film was observed in this work. The redox potential E0' = 0.46 V vs. NHE for first primary donor redox couple P/P+ was accurately measured from reversible CV or SWV peaks, which were quite close to those obtained from optic redox titration method. Reaction center (RC) in film was found re-constituted in such an ordered way that the orientation of RC favored the electron transfer in film. Thus, the protein electroactivity seems to be turned on in this artificial biomimic thin film. Furthermore, RC in the film features a photo-induced redox-peak fluctuation, suggesting an intact and functional state for RC in such film. Redox peaks were also found dependent of pH, implying a proton-coupled electron transfer occurring in film. Charge recombination was observed accompanied with change of electrochemical driving force. Electrochemical model assuming several classes of electroactive sites in the films on the electrode with a dispersion of standard potentials successfully fits SWV experimental data at different pulse height and frequency
Beschreibung:Date Completed 14.06.1999
Date Revised 03.11.2019
published: Print
Citation Status MEDLINE
ISSN:0302-4598