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01000naa a22002652 4500 |
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NLM100092950 |
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DE-627 |
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20231222115429.0 |
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tu |
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231222s1999 xx ||||| 00| ||eng c |
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|a pubmed24n0334.xml
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|a (DE-627)NLM100092950
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|a (NLM)10075864
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Rubinstein, D B
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Autoantibodies to leukocyte alphaMbeta2 integrin glycoproteins in HIV infection
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| 264 |
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1 |
|c 1999
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 13.04.1999
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| 500 |
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|a Date Revised 17.11.2004
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a Copyright 1999 Academic Press.
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| 520 |
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|a HIV infection is often associated with polyclonal B-cell activation, autoantibodies, and clinically evident autoimmune disease. Because neutropenia and anti-neutrophil autoantibodies are common clinical features of HIV disease, we studied a series of HIV+ patients to determine whether anti-alphaMbeta2 integrin (MAC-1) specific anti-neutrophil autoantibodies occur in HIV disease, as we have shown to occur in patients with immune neutropenia not associated with HIV. Two new assays specific for anti-alphaMbeta2 IgG were developed to carry out these studies: an ELISA method using affinity-purified alphaMbeta2 integrin protein, and a flow cytometry method using subclones of the 293 human fetal kidney cell line, stably transfected with cDNAs for the alphaM and/or beta2 integrin subunits. In studies of the sera of 20 untreated HIV+ individuals, anti-alphaMbeta2 activity was detected in 9 (45%) by one or the other of these assays and in 5 (25%) by both assays. Seven of the 20 HIV+ study subjects had unexplained neutropenia, and of these, 6 (86%) were positive for anti-alphaMbeta2 autoantibodies. Our findings indicate that anti-alphaMbeta2 integrin autoantibodies are frequent in HIV+ individuals, particularly when unexplained neutropenia is also present, and raise the possibility that these autoantibodies may have a role in the acquired neutrophil dysfunction and increased risk of nonopportunistic bacterial infections observed in HIV disease
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a Autoantibodies
|2 NLM
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| 650 |
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7 |
|a Glycoproteins
|2 NLM
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| 650 |
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7 |
|a Macrophage-1 Antigen
|2 NLM
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| 700 |
1 |
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|a Farrington, G K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a O'Donnell, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hartman, K R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wright, D G
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 90(1999), 3 vom: 01. März, Seite 352-9
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:90
|g year:1999
|g number:3
|g day:01
|g month:03
|g pages:352-9
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 90
|j 1999
|e 3
|b 01
|c 03
|h 352-9
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