Modified miniprep method for the rapid recovery of episomes from transfected breast epithelial cells
Episomal vectors such as pCEP4 are useful in expression cloning because they can replicate in both prokaryotes and eukaryotic cells. We have found a rapid and efficient means of extracting them from transfected MCF-10A nonmalignant human breast epithelial cells. We show that a plasmid miniprep proto...
| Veröffentlicht in: | BioTechniques. - 1988. - 26(1999), 2 vom: 01. Feb., Seite 276-80, 282 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1999
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Technical Report DNA, Complementary Deoxyribonucleases EC 3.1.- |