Fusion proteins could generate false positives in peptide phage display
Fusion proteins are frequently used in the functional characterization of newly discovered proteins and to identify interacting partners. In our study of hPTP1E, a cytosolic protein tyrosine phosphatase, we used glutathione S-transferase (GST)-fusion protein of the second PDZ domain to identify inte...
Veröffentlicht in: | BioTechniques. - 1988. - 26(1999), 1 vom: 01. Jan., Seite 142-9 |
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Weitere Verfasser: | , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1999
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Oligopeptides Peptide Library Recombinant Fusion Proteins Glutathione Transferase EC 2.5.1.18 PTPN13 protein, human EC 3.1.3.48 Protein Tyrosine Phosphatase, Non-Receptor Type 13 |