Long RT-PCR Amplification of the entire coding sequence of the polycystic kidney disease 1 (PKD1) gene
Characterization of mutations of the PKD1 gene has been limited by the fact that three-fourths of this gene at its 5' end is homologous to sequences of at least three other genes on the same chromosome. We have therefore developed a method of long reverse transcription PCR for selective amplifi...
| Veröffentlicht in: | BioTechniques. - 1991. - 26(1999), 1 vom: 01. Jan., Seite 126-32 |
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| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1999
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA, Complementary Proteins RNA, Messenger TRPP Cation Channels polycystic kidney disease 1 protein DNA Restriction Enzymes EC 3.1.21.- |