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|a eng
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| 100 |
1 |
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|a Nadin-Davis, S A
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Site-directed mutagenesis of large plasmids
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| 264 |
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1 |
|c 1998
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 11.03.1999
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| 500 |
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|a Date Revised 28.09.2018
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for site-directed mutagenesis of large (> 10 kb) plasmids. Application of this protocol to the generation of mutant rabies virus glycoproteins expressed by the baculovirus/insect cell system illustrates the usefulness of this approach in facilitating structure-function relationships in this important eukaryotic expression system
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| 650 |
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4 |
|a Technical Report
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a DNA Primers
|2 NLM
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| 650 |
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7 |
|a Glycoproteins
|2 NLM
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| 700 |
1 |
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|a Chang, S C
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 25(1998), 6 vom: 04. Dez., Seite 1014-9
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:25
|g year:1998
|g number:6
|g day:04
|g month:12
|g pages:1014-9
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