Retroviral gene transfer in chondrogenic limb bud micromass cultures
We report development of a model of retroviral gene transduction in high-density limb bud cell micromass culture. The replication competent avian retrovirus RCAS BP (A) carrying the human placental alkaline phosphatase gene (RCAS AP) was used as a marker for retroviral infection and spread. The fina...
| Publié dans: | BioTechniques. - 1991. - 24(1998), 4 vom: 15. Apr., Seite 660-6 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1998
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Gene Products, gag Collagen 9007-34-5 Alkaline Phosphatase EC 3.1.3.1 |