Bioluminometric method for real-time detection of reverse transcriptase activity

A simple and sensitive technique for detection of reverse transcriptase (RT) activity in real time has been developed. The technique is based on continuous detection of the inorganic pyrophosphate formed in the RT-catalyzed reaction by a luminometric method. The technique has been used for continuou...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 24(1998), 2 vom: 01. Feb., Seite 302-6
1. Verfasser: Karamohamed, S (VerfasserIn)
Weitere Verfasser: Ronaghi, M, Nyrén, P
Format: Aufsatz
Sprache:English
Veröffentlicht: 1998
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Deoxyribonucleotides Dideoxynucleotides Diphosphates Reverse Transcriptase Inhibitors Thymine Nucleotides DNA 9007-49-2 RNA-Directed DNA Polymerase mehr... EC 2.7.7.49 2',3'-dideoxythymidine triphosphate IA883WYU21