Isolation of full-length RNA from a thermophilic cyanobacterium

Isolation of full-length mRNA without degradation is critical in the study of in vivo gene regulation and transcription, cDNA synthesis and reverse transcription (RT)-PCR. It is particularly difficult to isolate full-length mRNA from thermophiles, which have higher turnover rates of mRNA degradation...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 23(1997), 5 vom: 07. Nov., Seite 904-6, 908, 910
1. Verfasser: Luo, X Z (VerfasserIn)
Weitere Verfasser: Stevens, S E Jr
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report Journal Article Guanidines Pentanols RNA, Bacterial RNA, Messenger Ribonucleosides Thiocyanates Vanadium Compounds mehr... Phenol 339NCG44TV guanidine thiocyanate 593-84-0 Mercaptoethanol 60-24-2 Chloroform 7V31YC746X isopentyl alcohol DEM9NIT1J4 Ribonucleases EC 3.1.- Lithium Chloride G4962QA067