Cloning and assembly of PCR products using modified primers and DNA repair enzymes
We present a method for the creation of ligatable 3' overhangs by the incorporation of a modified base, uracil, at a specific position in the PCR primer and subsequent treatment with the DNA-modifying enzyme uracil DNA glycosylase and then either T4 endonuclease V or human apurinic/apyrimidinic...
| Veröffentlicht in: | BioTechniques. - 1993. - 23(1997), 5 vom: 07. Nov., Seite 858-62, 864 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1997
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Technical Report Journal Article DNA Primers Escherichia coli Proteins Viral Proteins Uracil 56HH86ZVCT Chloramphenicol O-Acetyltransferase mehr... |