Calibration and storage of DNA competitors used for contamination-protected competitive PCR

Calibration and storage of DNA competitors used for contamination-protected competitive PCR

DNA fragments used as standards in competitive PCR were precisely calibrated using HPLC and commercially available DNA molecular mass markers. The accuracy of calibration was reflected by data that differed by only 2% from the mean when two independently purified and calibrated competitor preparatio...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 23(1997), 4 vom: 15. Okt., Seite 722-6
1. Verfasser: Köhler, T (VerfasserIn)
Weitere Verfasser: Rost, A K, Remke, H
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ATP-Binding Cassette Transporters DNA, Viral Multidrug Resistance-Associated Proteins RNA, Messenger Solutions DNA 9007-49-2 Glyceraldehyde-3-Phosphate Dehydrogenases mehr... EC 1.2.1.- Deoxyribonuclease HindIII EC 3.1.21.- DNA Glycosylases EC 3.2.2.- N-Glycosyl Hydrolases Uracil-DNA Glycosidase