In-cell assembly of scFv from human thyroid-infiltrating B cells

In-cell assembly of scFv from human thyroid-infiltrating B cells

The construction of a large library of single-chain Fv (scFv) antibody fragments involves a random assortment of heavy and light chains. Although useful for the production of recombinant antibodies, this method is not adapted to the study of the autoantibody repertoire formed in vivo during autoimmu...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 23(1997), 3 vom: 13. Sept., Seite 518-24
1. Verfasser: Chapal, N (VerfasserIn)
Weitere Verfasser: Bouanani, M, Embleton, M J, Navarro-Teulon, I, Biard-Piechaczyk, M, Pau, B, Peraldi-Roux, S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers Immunoglobulin Fragments Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin Variable Region immunoglobulin Fv