PNA-mediated purification of PCR amplifiable human genomic DNA from whole blood
A simple and rapid procedure whereby human genomic DNA can be purified in a PCR amplifiable form from whole blood is described. In a first step, human genomic DNA is hybridized in solution to a biotinylated peptide nucleic acid (PNA), which forms a high-affinity triplex with A7 sequence motifs in th...
| Veröffentlicht in: | BioTechniques. - 1993. - 23(1997), 3 vom: 13. Sept., Seite 512-7 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1997
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article CFTR protein, human Nucleic Acids Peptides Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6 Histidine 4QD397987E Biotin 6SO6U10H04 mehr... |