Analysis of assembly of synthetic antibody fragments : expression of functional scFv with predefined specificity
In this work, we have investigated parameters important for the assembly of synthetic genes encoding antibody fragments. These genes are constructed from a set of overlapping single-stranded oligonucleotides (primers), which are assembled into a gene sequence in a one-step process using PCR. Using t...
| Veröffentlicht in: | BioTechniques. - 1993. - 23(1997), 3 vom: 13. Sept., Seite 500-3 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1997
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Technical Report Journal Article DNA Primers Immunoglobulin Fragments Immunoglobulin Heavy Chains Oligonucleotides immunoglobulin Fv DNA 9007-49-2 |