Application of oligonucleotide activation to restriction endonuclease NarI

Restriction endonuclease NarI cleaves DNA using a two-site mechanism, placing it in the Type IIe class of restriction endonucleases. Although these enzymes have very useful recognition sequences, the two-site mechanism limits the practical application. Site preferences often cause incomplete substra...

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Veröffentlicht in:BioTechniques. - 1993. - 22(1997), 6 vom: 15. Juni, Seite 1166-8
1. Verfasser: Senesac, J H (VerfasserIn)
Weitere Verfasser: Romanin, J K
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article DNA, Viral Oligodeoxyribonucleotides Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4 GGCGCC-specific type II deoxyribonucleases