Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion

Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion

The precise quantification of rare mRNA copies from intronless genes by reverse transcription polymerase chain reaction (RT-PCR) requires the complete removal of genomic DNA because discrimination of cDNA and DNA amplification products by differing sizes of PCR products is not possible. Elimination...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 22(1997), 6 vom: 15. Juni, Seite 1128-32
1. Verfasser: Bauer, P (VerfasserIn)
Weitere Verfasser: Rolfs, A, Regitz-Zagrosek, V, Hildebrandt, A, Fleck, E
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Pyruvate Dehydrogenase Complex RNA, Messenger Manganese 42Z2K6ZL8P Taq Polymerase EC 2.7.7.- RNA-Directed DNA Polymerase EC 2.7.7.49 DNA-Directed DNA Polymerase mehr... EC 2.7.7.7 Deoxyribonuclease I EC 3.1.21.1 Magnesium I38ZP9992A