High-throughput RT-PCR analysis of multiple transcripts using a microplate RNA isolation procedure

High-throughput RT-PCR analysis of multiple transcripts using a microplate RNA isolation procedure

We have developed a high-throughput, multiplex reverse transcription PCR (RTPCR) assay that is suitable for the analysis of medium-to low-copy cellular RNA transcripts from small numbers of cells (10(4)). High throughput was attained by utilizing microplate-based RNA extraction and RTPCR protocols,...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 22(1997), 6 vom: 15. Juni, Seite 1107-13
1. Verfasser: Su, S (VerfasserIn)
Weitere Verfasser: Vivier, R G, Dickson, M C, Thomas, N, Kendrick, M K, Williamson, N M, Anson, J G, Houston, J G, Craig, F F
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Benzothiazoles DNA Primers Diamines Fluorescent Dyes Imidazoles Interleukin-1 Lactones Lipopolysaccharides Macrolides mehr... Organic Chemicals Quinolines RNA, Messenger Solvents Thiazoles Tumor Necrosis Factor-alpha SYBR Green I 163795-75-3 6-(4-fluorophenyl)-2,3-dihydro-5-(4-pyridinyl)imidazo(2,1-b)thiazole 9R6QDF1UO7 Glyceraldehyde-3-Phosphate Dehydrogenases EC 1.2.1.- monorden I60EH8GECX