Cloning DNA fragments between two adjacent/overlapping restriction sites using a "positive stuffer"
Here we describe a solution to a common problem encountered in recombinant DNA cloning when directional cloning of a DNA fragment into a predetermined plasmid requires the use of restriction enzymes with adjacent or overlapping recognition sites. In preparing the double-digested plasmid, only one en...
| Veröffentlicht in: | BioTechniques. - 1993. - 22(1997), 5 vom: 25. Mai, Seite 912-5 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1997
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report Journal Article Viral Proteins gene 43 protein, Enterobacteria phage T4 DNA 9007-49-2 DNA-Directed DNA Polymerase EC 2.7.7.7 DNA Restriction Enzymes EC 3.1.21.- mehr... |