Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity

The ratio of absorbance at 260 and 280 nm (the A260/280 ratio) is frequently used to assess the purity of RNA and DNA preparations. Data presented in this report demonstrate significant variability in the RNA A260/280 ratio when different sources of water were used to perform the spectrophotometric...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 22(1997), 3 vom: 15. März, Seite 474-6, 478-81
Auteur principal: Wilfinger, W W (Auteur)
Autres auteurs: Mackey, K, Chomczynski, P
Format: Article
Langue:English
Publié: 1997
Accès à la collection:BioTechniques
Sujets:Technical Report Journal Article Phosphates Proteins Water 059QF0KO0R RNA 63231-63-0 DNA 9007-49-2