Labeling of RNA transcripts of eukaryotic cells in culture with BrUTP using a liposome transfection reagent (DOTAP)
Bromouridine-triphosphate (BrUTP), when introduced into eukaryotic cells in culture, substitutes for UTP during transcription, thereby labeling pre-mRNA for detection by immunochemical methods. In earlier studies, BrUTP was internalized by means of microinjection or by exposing isolated nuclei or pe...
| Publié dans: | BioTechniques. - 1991. - 22(1997), 2 vom: 31. Feb., Seite 308-12 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1997
|
| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Fatty Acids, Monounsaturated Fluorescent Dyes Liposomes Quaternary Ammonium Compounds RNA Precursors RNA, Messenger 5-bromouridine triphosphate 3398-50-3 plus... |