Inhibition of PCR amplification by a point mutation downstream of a primer
A T-->C point mutation is shown to specifically inhibit PCR amplification when compared to wild-type controls in exon H of the factor IX gene. Multiple primers of different lengths and locations were designed to examine this phenomenon. The experiments suggest that poor annealing and/or extension...
| Veröffentlicht in: | BioTechniques. - 1991. - 22(1997), 2 vom: 31. Feb., Seite 292-4, 296, 298, passim |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1997
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA Primers Factor IX 9001-28-9 DNA 9007-49-2 |