Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions
Using a cell-bound immunogen, we have generated a monoclonal antibody, 3D5, that recognizes carboxy-terminal oligo-histidine tags (His tags) on a wide variety of proteins. From this monoclonal antibody, we have generated a single-chain fragment of the variable domains (scFv), a dimeric scFv-alkaline...
Veröffentlicht in: | BioTechniques. - 1993. - 22(1997), 1 vom: 07. Jan., Seite 140-9 |
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1. Verfasser: | |
Weitere Verfasser: | , , , , , , , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1997
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article Antibodies, Monoclonal Immunoglobulin Fragments Indicators and Reagents Recombinant Proteins Histidine 4QD397987E |