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tu |
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231222s1997 xx ||||| 00| ||eng c |
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Aliev, T K
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Deoxyribonuclease treatment improves the homogeneity of single-stranded DNA preparations
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| 264 |
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1 |
|c 1997
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 25.03.1997
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a The isolation of single-stranded (ss) phagemid DNA using standard protocols often results in impure preparations, which contain undesirable quantities of chromosomal and/or double-stranded (ds) phagemid DNA. Here we report a simple and efficient method for elimination of virtually all dsDNA by incubation of phagemid viral particles with deoxyribonuclease I. In addition to analyzing the ratio of linear-to-circular topological forms of ssDNA after deoxyribonuclease I treatment, we verified that no decrease in transformation efficiency occurred and demonstrated that ssDNA molecules covered by capsid proteins remained intact following such treatment
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| 650 |
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|a Technical Report
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|a Journal Article
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| 650 |
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7 |
|a DNA, Single-Stranded
|2 NLM
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| 650 |
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7 |
|a DNA, Viral
|2 NLM
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| 650 |
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7 |
|a Deoxyribonucleases
|2 NLM
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| 650 |
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7 |
|a EC 3.1.-
|2 NLM
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| 700 |
1 |
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|a Panina, A A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Korobko, V G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Varfolomeyev, S D
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 22(1997), 1 vom: 07. Jan., Seite 105-8, 110
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:22
|g year:1997
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|g day:07
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|g pages:105-8, 110
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