DNA sequencing with [alpha-33P]-labeled ddNTP terminators : a new approach to DNA sequencing with Thermo Sequenase DNA polymerase
A new approach to DNA sequencing is described. The method is based on the use of [alpha-33P]-labeled dideoxyribonucleoside triphosphate terminators and Thermo Sequenase DNA polymerase in cycle sequencing. Thermo Sequenase DNA polymerase incorporates ddNTPs as efficiently as dNTPs, allowing the use o...
| Publié dans: | BioTechniques. - 1991. - 21(1996), 6 vom: 15. Dez., Seite 1132-7 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1996
|
| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Codon, Terminator DNA Primers DNA, Bacterial Reagent Kits, Diagnostic DNA-Directed DNA Polymerase EC 2.7.7.7 |